![]() Weigh out 292 g NaCl, and add ddH2O to one liter final volume. Adjust pH to 5.0 with glacial acetic acid. This solution is helpful for high GC content template in PCR.ĭissolve 408 g sodium acetate(x3H2O) in 800 ml ddH2O. ![]() Sterilize by 0.22 um filter, and store at -20¡ãC. Store at -20¡ãC.ĭissolve 29.275 g betaine in final volume of 50 ml sterile ddH2O. The 10x solution can be diluted as 1:10 with sterile ddH20 to prepare a 1xworking solution.ĭissolve 174 mg PMSF in 10 ml isopropanol. (The final pH will be ~7.3.) Autoclave and store at room temperature. There is no need to adjust pH for this solution. After solidification, open the dishes in the hood and dry the plate for about 1 hour.įor ampicillin, use 0.5 ml of 1000x amp stock per 500 ml media.įor kanamycin, use 1 ml of 500x kan stock per 500 ml mediaġ0 x PBS (Phosphate buffered saline) Chemicalĭissolve in ddH2O to a final volume of 1 liter. Add antibiotic if desired, and quickly pour into sterile petri dishes until dish surface is completely covered. Autoclave immediately, and cool to 50¡ãC in water bath. Weigh out 32 g pre-made LB agar mix, which is composed of:Īdd magnetic stirring bar, add 1 liter ddH2O. Adjust pH to 7.5 with potassium hydroxide (KOH) and store at 4¡ãCĭissolve 0.5 g kanamycin in 10 ml of sterile ddH2O. Filter sterilize (0.22 um filter), and store at room temperature.Īdd 23.83 g HEPES (Sigma H-3375) to 100 mo of ddH2O. ![]() Wrap in foil, store in dark.ĭissolve 200 g (D)-glucose in a final volume of 500ml ddH2O. Filter with 0.5 micron filter and autoclave.Īdd 100 mg EtBr (Sigma E-8751) to 10 ml ddH2O. Note that the EDTA won't completely dissolve until the pH is around 8. Add the last few grams slowly to avoid overshooting the pH. Add about 20g of NaOH pellets while stirring to bring the pH to 8.0. Store at room temperature.Ġ.5 M EDTA, pH 8.0 (disodium ethylenediamine tetraacetate):Īdd 186.1 g EDTA (Sigma # E-5134) to 800 ml of ddH20. Store at -20¡ãCĢ5mg bromophenol blue (0.25%), 25mg xylene xyanol (0.25%), 4g sucrose (40%), adjust volume to 10 ml with H2O. Filter through a 0.22 um filter, Store at -20¡ãC.ĭissolve 15.45 g DTT in 100 ml H2O. Vortex until fully dissolved, and store at 4¡ãC.ĭissolve 1 g ampicillin in 10 ml of sterile ddH2O. Weight 1 g of ammonium persulphate in 15 ml Corning Tube and add about 9.5 ml of sterile ddH2O. Cool at 60 ¡ãCĪnd add 20 ml ethidium bromide solution (10 mg/ml) before use. Wrap bottle in foil, and store in dark at 4¡ãC.ĭissolve agarose completely by microwaving. Check pH: if above 7.0, add HCI until at or below 7.0. ![]() Heat to 37¡ãC until completely dissolved. Weigh out the components above, and begin dissolve them in about 60% of the final volume of ddH2O. TAE (Tris/acetate/EDTA) electrophoresis buffer SSC (sodium chloride/sodium citrate), 20x PMSF (phenylmethylsulphonyl fluoride), 100 mM ![]()
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